Antiplasmodial activity of selected medicinal plants. Plant extracts showed moderate to good antiparasitic effects. The 50% inhibitory concentration ic50 values of the crude aqueous extracts were found to be 2. Evaluation of the antiplasmodial properties of namibian. The plant extract was prepared by maceration in methanol. Various phytotoxins released by necrotropic plant pathogenic fungi may inhibit the plantlike metabolic pathways in the apicoplast, a chloroplastlike organelle, essential for the survival of apicomplexan parasites, such as plasmodium which cause malaria in humans. Larvicidal, antipyretic and antiplasmodial activity of.
Further studies are warranted to explore its efficacies and lead compounds of these three plant extracts for the. The plant leaves were successively extracted into three 3 extract forms aqueous extract, ethanolic extract and hydroethanolic 50. Sixtynine extracts from 11 plant species were prepared and screened for in vitro activity against p. Nmrbased metabolomic study of medicinal plants used against malaria and the isolation of bioactive alkaloids. Evaluation of the antiplasmodial activity of extracts of. The results showed antioxidant activity using dpph were found to be increased in a concentration dependent.
Among the various extracts, the lowest 50% inhibitory concentrations ic50 of 24. Antioxidant activity of the plant extracts were characterized by using dpph free radical scavenging method. The in vitro cytotoxicity assay of the 6 plant crude extracts using kb cells indicated that t. Plant extract achieved a maximum antioxidant activity of 4% within 40 min. Antiplasmodial activity and toxicity of crude extracts. In vitro and in vivo antiplasmodial activities of gynura. Plant extracts were correspondingly subjected to an antiproliferative bioassay against mammalian skeletal myoblast cells. Okundoperoxide, a bicyclic cyclofarnesylsesquiterpene. The tannins extracts showed moderate antiplasmodial activity on both p. Antiplasmodial compounds from leaves of dodonaea angustifolia. Antiplasmodial activity of vernonia adoensis aqueous, methanol.
The cytotoxic activity of the extracts of cocculus hirsutus on mcf7 cells was investigated in vitro through mtt assay. Antiplasmodial activity of methanol leaf extract of citrus. Nmrbased metabolomic study of medicinal plants used. This is the first scientific report to document significant antiplasmodial activity of extracts from t. Various extracts obtained from 19 plants collected in kenya were tested for antiplasmodial activity. Data analysis using twoway anova to determine the significant interactions between plant extracts and plasmodic growth. Antiplasmodial, antitrypanosomal, antileishmanial and. Evaluation of antiplasmodial activity of extracts and constituents. Antifungal activity of six botanical extracts was assayed and the effect of plant extracts on the growth of fungal strain f. In vitro antiplasmodial activity and toxicity assessment of plant. The present study investigates the antimalarial activity of the methanolic extracts. Data analysis using twoway anova to determine the significant interactions between plant extracts and. The proliferation and resistance of microorganisms area serious threat against humankind and the search for new therapeutics is needed.
The results of antimicrobial activity showed that water and ethanol crude extracts were active on all the strains of pathogens tested at different concentrations, with ethanol extracts exerting more activity. Promising antiplasmodial activity was found in the extracts from two plants, phyllanthus emblica leaf. Three plant extracts caused 100% inhibition of the parasite growth at concentrations of plant material. Pdf antiplasmodial activity and phytochemical analysis. Antiplasmodial activity of solvent fractions of methanolic. The use of plant extracts with known antimicrobial properties, can be of great significance in therapeutic treatments. The selected plants, acokanthera oppositifolia lam apocynaceae is a species encountered in eastern. Evaluation of the antiplasmodial activity and lethality of. Thus, there is a need for alternative source of medicine in malaria treatment and prevention. Pdf in vitro antimalarial activity of medicinal plant extracts against. Promising antiplasmodial activity was found in the extracts from two plants, phyllanthus emblica leaf 50% inhibitory concentration ic 3d7.
In this study the antiplasmodial effect of the leaf crude extract of v. Antiplasmodial activity was assessed 1 corresponding author. Plant extract exhibited no signs of toxicity on mice at a dose of 5000. The plants are usually used to treat the malaria disease, and the people commonly use the extract of this plants for this purpose 5. Of these, extracts from artabotrys crassifolius, pericampylus glacus and leuconotis eugeniifolia showed promising antiplasmodial activities at ic 50 of 15. In vitro antiplasmodial assays on plasmodium falciparum in human red blood cell culture. Antiplasmodial activity of some medicinal plants used in. Antiplasmodial activity of extracts of tridax procumbens and phyllanthus amarus in in vitro plasmodium falciparum culture systems.
Preliminary evaluation of selected medicinal plants for antiplasmodial activity 40 table 3. Antiplasmodial activities of crude extract and solvent fractions were. Both plant extracts also demonstrated antiplasmodial activity against the chloroquine resistant plasmodial parasites. In vitro antiplasmodial activity of dysoxylum caulostachyum miq. Cytotoxycity and antiplasmodial activity of phenolic. All plant samples displayed various antiplasmodial activity. All extracts and pure compounds were tested for antiplasmodial activity in which the hexane bark extract showed highest activity with ic 50 0. The results of the 12 fractions of three of the bioactive plant extracts in the in vitro antiplasmodial assay. The structures of isolated compounds were elucidated using chemical and spectroscopic methods. In vitro antiplasmodial and cytotoxic activities of. From the studies conducted on the plant until now, it is known that the methanolic root extract of the plant has schizonticidal activity as high as 84. Seven of the eleven extracts exhibited significant antiplasmodial activity ic 50 edulis, c. Annickia enantia kummeriae annonaceae, artemisia annua asteraceae, pseudospondias microcarpa anacardiaceae, drypetes natalensis euphorbiaceae, acridocarpus chloropterus malpighiaceae, maytenus senegalensis celastraceae and.
In the in vivo evaluation of the antiplasmodial activity of the plant extracts was employed against. The plant extracts analysed in this study presented an average antiplasmodial activity 58%. Antiplasmodial activity of medicinal plant preparations. A preliminary screening of potentially antimalarial plants. Six methanol meoh extracts and one chloroform extract showed in vitro antiplasmodial activity against the d6 plasmodium falciparum strain, while only three. The extracts of experimental plants were evaluated for their antiplasmodial activity against 3d7 and indo strains of p. Summary the in vitro antiplasmodial activities of 14 plant species traditionally used in central america for the treatment of malaria or fever were evaluated.
The in vitro antiplasmodial activity of seven etoh extracts and twenty fractions from the partition of the initial ethanolic extracts from seven african medicinal plants used in the democratic republic of congo dr congo for the treatment of malaria was evaluated. Thus, the present study aims at evaluating the invivo antiplasmodial activity of solvent fractions of methanolic root extract of. Antiplasmodial activity of vernonia adoensis aqueous. In sudan, out of 21 compounds isolated from 9 medicinal plants used in traditional medicine, only gedunin and quercetin showed ic50 of 1. The purpose of this study was to discover antimalarial compounds from necrotropic plant pathogenic fungi. In vitro antiplasmodial activity of extracts from cassia fistula tissues.
For quality control, high performance thin layer chromatography hptlc was performed on extracts. The plant extracts were obtained from forest research institute malaysia frim. The in vitro antiplasmodial activity of seven etoh extracts and twenty fractions from the partition of the initial ethanolic extracts from seven african medicinal plants used in the democratic. Antiplasmodial activity and phytochemical analysis of extracts from selected ugandan medicinal plants. Other related study reported that screening of the appropriate extracts of ochrosia maculata elicited. In vitro antiplasmodial activity of extracts and fractions. The present report describes the antiplasmodial and anticancer activities of samples isolated from the methanol extract of albizia zygia mimosaseae.
The in vitro antiplasmodial activity and cytotoxicity of extracts from jatropha tanjorensis leaves was evaluated. Antiplasmodial activity of methanol leaf extract of citrus aurantifolia christm swingle. Bacterial diversity and community structure in typical plant rhizosphere. In previous study on antimalarial plants from mali 31 crude extracts of a. The methanolic extract phyllanthus niruri stone breaker showed the maximum activity against staphylococcus sp. Results in vitro antiplasmodial activity and cytotoxicity of aqueous extracts all five aqueous extracts showed activity against the c h l o r o q u i n e s e n s i t i v e 3 d 7 p. The methanolic leaf extract of ricinus communis is a low toxic plant with antiplasmodial activity which can be exploited as a potential source of new antimalarial drug to combat malaria. In vitro antiplasmodial activity the plant extracts were tested in triplicate against chloroquine sensitive cqs strain of p. In vitro and in vivo antimalarial activity of plants from. This suggests that leaves should be preferred over the whole plant and extraction should be made in ethyl acetate for enriching antiplasmodial molecules. Lipophilic extracts of piper hispidum, siparuna andina, s.
Moreover, all plant extracts were found to have in vitro antiplasmodial activity. But all the mice treated with cq survived for 3 months additional file 1. Preliminary evaluation of selected medicinal plants for. In vitro assessment of the antiplasmodial activity of three plants. In vitro antiplasmodial activity and phytochemicals. In vivo and in vitro antimalarial activity of vernonia. Thus, it is worth documenting the ethnobotanical information and testing the antiplasmodial activity of plant extracts. Also, the methanol plant extracts were more potent than their corresponding aqueous extracts. In vitro antimalarial activity of medicinal plant extracts against plasmodium falciparum.
Antimalarials from plant pathogenic fungi by mallika. In vitro antiplasmodial activity of aqueous and organic extracts from moringa ovalifolia was measured using parasitaemia posttreatment with plant extracts as well as the ic50 values. In vitro antiplasmodial activity and cytotoxicity of. The data revealed that significant reduction in growth of fungal strain f.
Antimalarial and antiplasmodial activity papaya leaves are made into tea as a treatment for malaria. Antibacterial activity of alkaloid extracts from ochrosia. The results from the in vitro antiplasmodial testing of 7 etoh extracts and 20 fractions from the partition of their respective initial etoh extracts are presented in table 2. The antiplasmodial activities were carried out using the pldh assay to plasmodium falciparum d10 strain sensitive strain while the cytotoxic activities were carried out towards madindarby bovine kidney mdbk cells using mtt assay. Herein, the in vitro and in vivo antiplasmodial activity and cytotoxicity of medicinal plant extracts were investigated. Continuous in vitro cultures of asexual erythrocytes stages of p. Drugs and plant extracts were then placed in 96well flat bottom tissue culture grade plates.
Antibacterial activity of plants 719 effective extracts are considered, e. The two most active extracts that produced 100% inhibition of the parasite growth at concentration of plant material. The current study was undertaken to evaluate the antiplasmodial activity and toxicity of the organic extracts of cassia alata. European scientific journal april 2016 edition vol. Evaluation of antimalarial activity of hydromethanolic crude extract a. The antiplasmodial activity of the extracts and pure compounds was evaluated using the standard peters fourday suppressive method. Antiplasmodial properties of some malaysian medicinal plants.
M as antiplasmodial activity when tested in vitro against plasmodium falciparum. The emergence and spread of plasmodium falciparum with resistance to chloroquine cq, the safest and cheapest antimalarial drug, coupled with the increasing cost of alternative drugs especially in developing countries have necessitated the urgent need to tap the potential of plants for novel antimalarials. With regard to the ethanolic extract a, a high antiplasmodial activity ic 50 pdf antiplasmodial activity of plant extracts used in west african traditional medicine five plants originating from ivory coast were selected after an ethnobotanical survey, alchornea. Quantitative assessment of antiplasmodial activity in. Plant extract showed concentration dependent antioxidant activity and ferric reducing power. Antimicrobial activity of selected medicinal plants. Antiplasmodial activity of the ethnobotanical plant.
The plant leaves were successively extracted into three 3 extract forms including aqueous extract, ethanolic extract and hydroethanolic 50. Facilitate digestion the leaves of the papaya plants contain. Evaluation of antiplasmodial activity of medicinal plants. John oludele olanlokun, oluwole moses david, anthony jide afolayan. Antiplasmodial activity of aqueous leaf extract of. Antioxidant and in vitro cytotoxic activity of extracts of.
In vitro antiplasmodial activity of central american. In vitro antiplasmodial activities and synergistic. In nigeria, malaria is a major public health problem and there are high cost of the effective antimalarial drugs, poor quality drugs and increased emergence of plasmodial resistance. Selected plants being used in indian traditional medicine to treat malaria and malarialike symptoms plant name, family, part used common name method of preparation referencs adhatoda zeylanica medic. In vitro antiplasmodial activity and prophylactic potentials of extract. Antibacterial activity of plant extracts 247 brazilian journal of microbiology 2000 31. Pdf in vitro antimalarial activity of medicinal plant. The antiplasmodial, antitrypanosomal and antileishmanial activity of 25 plant extracts obtained from seven tanzanian medicinal plants. The antimalarial potential of aqueous leaf extracts of cymbopogon citratus was investigated in this present study. The antiplasmodial activity of extracts of these plants was evaluated in vitro using the multiresistant strain w2 of plasmodium falciparum. This study revealed for the first time the antiplasmodial activity of the plant c. Pdf antiplasmodial activity and phytochemical analysis of extracts. Antiplasmodial potential of medicinal plant extracts from.